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KMID : 0357920030370060379
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Korean Journal of Pathology
2003 Volume.37 No. 6 p.379 ~ p.383
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Comparison of Ziehl-Neelsen Stain and TB-PCR on Detection of Mycoboctedum tuberculosis in Formalin-fixed, Paraffin-embedded Tissues of Chronic Granulomatous Inflammation
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Cho Min-Sun
Sung Sun-Hee
Han Woon-Sup
Lee Shi-Nae
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Abstract
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Background: TB-PCR is a faster and more sensitive method to detect mycobacterium than acid-fast bacilli (AFB) stain, which is laborious and time consuming. We compared the sensitivity and specificity of AFB stain and TB-PCR and examined the possibility of TB-PCR as a confirmative test without AFB stain in the diagnosis of tuberculosis.
Methods: We performed Ziehl-Neelsen stain and nested PCR using a commercially available TB-PCR kit amplifying IS61 10 sequence in 81 cases of paraffin-embedded tissues diagnosed as chronic granulomatous inflammation. In addition, we evaluated the morphology of granuloma and the presence of caseation necrosis.
Resuits: Of the 81 cases studied, 22(27.2%) and 40(49.4%) were positive for AFB stain and TB-PCR, respectively. Of 49 cases accompanying caseation necrosis, 19(38.8%)were AFB stain positive and 37(75.5%) were TB-PCR positive; a result that is comparable with that of other reports. Of the 22 AFB-positive cases, 2 were TB-PCR negative.
Conclusion: TB-PCR is very helpful for the diagnosis of tuberculosis in routinely processed, formalin-fixed, paraffin-embedded tissue samples. Nevertheless, AFB stain should continue to be performed at the same time.
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KEYWORD
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Chronic Granulomatous Diseases, Mycobacterium tuberculosis, Stains, PCR
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